Coding

Part:BBa_K4482003:Design

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-08-25)


Human IR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 963
    Illegal PstI site found at 2464
    Illegal PstI site found at 2730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 963
    Illegal PstI site found at 2464
    Illegal PstI site found at 2730
    Illegal NotI site found at 28
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 963
    Illegal BglII site found at 1532
    Illegal BamHI site found at 1878
    Illegal XhoI site found at 286
    Illegal XhoI site found at 2043
    Illegal XhoI site found at 3056
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 963
    Illegal PstI site found at 2464
    Illegal PstI site found at 2730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 963
    Illegal PstI site found at 2464
    Illegal PstI site found at 2730
    Illegal NgoMIV site found at 14
    Illegal NgoMIV site found at 2116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1744
    Illegal BsaI site found at 2054
    Illegal BsaI.rc site found at 221
    Illegal BsaI.rc site found at 796
    Illegal BsaI.rc site found at 1252
    Illegal BsaI.rc site found at 1574
    Illegal BsaI.rc site found at 3151
    Illegal BsaI.rc site found at 3326
    Illegal SapI site found at 267
    Illegal SapI.rc site found at 3333


Design Notes

This part came in a plasmid as a fusion protein with eGFP and didn't require any further cloning or modifications for the purpose of the project.


Source

This part was obtained as a plasmid from Addgene with code 22286.

References